| 质粒名称: | pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115267 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | SUMO bacterial expression vector |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6075 bp |
| 完整质粒大小: | 9564 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LbuCas13a,Cas13a from Leptotrichia buccalis C-1013-b |
| 物种来源: | Leptotrichia buccalis C-1013-b |
| 插入大小: | 3489 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | SUMO |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI was a gift from Feng Zhang (Addgene plasmid # 115267 ; http://n2t.net/addgene:115267 ; RRID:Addgene_115267) |
| 参考文献部分: | Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. 10.1126/science.aaq0179 PubMed 29449508 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI 9561 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses LbuCas13a from a SUMO-tagged bacterial expression
construct.
ACCESSION .
VERSION .
KEYWORDS pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9561)
AUTHORS Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang
F
TITLE Multiplexed and portable nucleic acid detection platform with Cas13,
Cas12a, and Csm6.
JOURNAL Science. 2018 Apr 27;360(6387):439-444. doi:
10.1126/science.aaq0179. Epub 2018 Feb 15.
PUBMED 29449508
REFERENCE 2 (bases 1 to 9561)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9561
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1..25
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 40..62
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 81..98
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 108..125
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS 138..221
/codon_start=1
/product="two Strep-Tag(R)II moieties connected by a linker
for enhanced binding to Strep-Tactin(R), an engineered form
of streptavidin (Schmidt et al., 2013)"
/label=Twin-Strep-tag
/translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
CDS 222..512
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/label=SUMO
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
primer_bind complement(4043..4061)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 4057..4104
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(4279..4307)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(4334..4352)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4420..4524
/gene="bla"
/label=AmpR promoter
CDS 4525..5385
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4743..4762)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5556..6144
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6045..6064
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6298..6315
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 6330..6469
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(6395..6414)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6555..6577
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(6571..6762)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(8074..9156)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 9118..9137
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9157..9234)
/gene="lacI"
/label=lacI promoter
primer_bind 9440..9459
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 9543..1
/label=T7
/note="T7 promoter, forward primer"
promoter 9543..9561
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
121 gcagccatat ggctagctgg agccatccgc agtttgaaaa aggtggtggt agcggtggtg
181 gttcaggtgg tagtgcatgg tcacaccctc agtttgagaa aatgtcggac tcagaagtca
241 atcaagaagc taagccagag gtcaagccag aagtcaagcc tgagactcac atcaatttaa
301 aggtgtccga tggatcttca gagatcttct tcaagatcaa aaagaccact cctttaagaa
361 ggctgatgga agcgttcgct aaaagacagg gtaaggaaat ggactcctta agattcttgt
421 acgacggtat tagaatccaa gctgatcaga cccctgaaga tttggacatg gaggataacg
481 atattattga ggctcacaga gaacagattg gtggatctat gaaggtgaca aaagtgggcg
541 gcatcagcca caagaagtat acctccgagg gcaggctggt gaagtctgag agcgaggaga
601 acaggaccga cgagcgcctg tccgccctgc tgaatatgag gctggatatg tatatcaaga
661 accccagctc caccgagaca aaggagaatc agaagcgcat cggcaagctg aagaagttct
721 tttccaacaa gatggtgtat ctgaaggaca atacactgtc tctgaagaat ggcaagaagg
781 agaacatcga ccgggagtac tctgagacag atatcctgga gagcgacgtg agagataaga
841 agaacttcgc cgtgctgaag aagatctacc tgaatgagaa cgtgaacagc gaggagctgg
901 aggtgttccg gaatgatatc aagaagaagc tgaacaagat caattctctg aagtacagct
961 ttgagaagaa caaggccaat tatcagaaga tcaacgagaa caatatcgag aaggtggagg
1021 gcaagagcaa gagaaatatc atctacgact actataggga gtccgccaag cgggacgcct
1081 acgtgagcaa cgtgaaggag gcctttgaca agctgtataa ggaggaggat atcgccaagc
1141 tggtgctgga gatcgagaat ctgaccaagc tggagaagta caagatcagg gagttctatc
1201 acgagatcat cggccgcaag aatgacaagg agaactttgc caagatcatc tacgaggaga
1261 tccagaacgt gaacaatatg aaggagctga tcgagaaggt gcctgatatg tccgagctga
1321 agaagtctca ggtgttctat aagtactatc tggacaagga ggagctgaac gataagaata
1381 tcaagtacgc cttctgccac tttgtggaga tcgagatgag ccagctgctg aagaactacg
1441 tgtataagag gctgtccaac atctctaatg acaagatcaa gcgcatcttt gagtatcaga
1501 atctgaagaa gctgatcgag aataagctgc tgaacaagct ggatacatac gtgagaaatt
1561 gtggcaagta taactactat ctgcaggacg gcgagatcgc cacctccgat ttcatcgccc
1621 ggaacagaca gaatgaggcc tttctgcgga acatcatcgg cgtgtctagc gtggcctact
1681 tctctctgag aaatatcctg gagacagaaa acgagaatga catcacaggc aggatgcgcg
1741 gcaagaccgt gaagaacaat aagggcgagg agaagtacgt gtctggcgag gtggataaga
1801 tctacaacga gaacaagaag aatgaggtga aggagaacct gaagatgttc tacagctatg
1861 actttaacat ggataacaag aatgagatcg aggacttctt tgccaatatc gatgaggcca
1921 tctcctctat caggcacggc atcgtgcact tcaacctgga gctggagggc aaggacatct
1981 tcgcctttaa gaatatcgcc ccaagcgaga tctccaagaa gatgtttcag aacgagatca
2041 atgagaagaa gctgaagctg aagatcttcc ggcagctgaa ctccgccaac gtgttcagat
2101 acctggagaa gtataagatc ctgaactacc tgaagcggac aagattcgag tttgtgaaca
2161 agaatatccc cttcgtgcct tcttttacca agctgtatag ccgcatcgac gatctgaaga
2221 actctctggg catctactgg aaaaccccta agacaaacga cgataataag acaaaggaga
2281 tcatcgacgc ccagatctat ctgctgaaga acatctacta tggcgagttc ctgaattact
2341 ttatgtctaa caatggcaac ttctttgaga tcagcaagga gatcatcgag ctgaacaaga
2401 atgacaagag gaacctgaaa accggcttct acaagctgca gaagtttgag gatatccagg
2461 agaagatccc aaaggagtac ctggccaata tccagtccct gtatatgatc aacgccggca
2521 atcaggacga ggaggagaag gacacataca tcgatttcat ccagaagatc ttcctgaagg
2581 gctttatgac ctacctggcc aacaatggcc gcctgtccct gatctatatc ggctctgacg
2641 aggagacaaa cacaagcctg gccgagaaga agcaggagtt cgataagttt ctgaagaagt
2701 acgagcagaa caataacatc aagatcccct atgagatcaa tgagttcctg agggagatca
2761 agctgggcaa catcctgaag tacacagagc gcctgaatat gttttatctg atcctgaagc
2821 tgctgaatca caaggagctg accaacctga agggcagcct ggagaagtac cagtccgcca
2881 acaaggagga ggccttctcc gaccagctgg agctgatcaa cctgctgaat ctggataata
2941 acagggtgac agaggacttt gagctggagg ccgatgagat cggcaagttc ctggacttta
3001 acggcaataa ggtgaaggat aacaaggagc tgaagaagtt cgacaccaat aagatctact
3061 ttgatggcga gaacatcatc aagcaccgcg ccttctacaa tatcaagaag tatggcatgc
3121 tgaacctgct ggagaagatc gccgacaagg ccggctacaa gatcagcatc gaggagctga
3181 agaagtattc caacaagaag aatgagatcg agaagaatca caagatgcag gagaacctgc
3241 accggaagta tgccaggcca cgcaaggatg agaagttcac agacgaggat tacgagagct
3301 ataagcaggc catcgagaac atcgaggagt acacccacct gaagaataag gtggagttta
3361 acgagctgaa tctgctgcag ggcctgctgc tgagaatcct gcacagactg gtgggctaca
3421 catccatctg ggagcgggac ctgcggttca gactgaaggg cgagtttccc gagaaccagt
3481 atatcgagga gatcttcaac tttgagaata agaagaacgt gaagtacaag ggcggccaga
3541 tcgtggagaa gtacatcaag ttctataagg agctgcacca gaatgacgag gtgaagatca
3601 acaagtatag ctccgccaat atcaaggtgc tgaagcagga gaagaaggat ctgtatatcc
3661 ggaactatat cgcccacttt aattacatcc ctcacgccga gatcagcctg ctggaggtgc
3721 tggagaacct gcggaagctg ctgagctatg acagaaagct gaagaatgcc gtgatgaagt
3781 ccgtggtgga tatcctgaag gagtacggct tcgtggccac atttaagatc ggcgccgaca
3841 agaagatcgg catccagacc ctggagtctg agaagatcgt gcacctgaag aacctgaaga
3901 agaagaagct gatgaccgat agaaatagcg aggagctgtg caagctggtg aagatcatgt
3961 tcgagtacaa gatggaggag aagaagtccg agaactaagg ccgcactcga ggcccgaaag
4021 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
4081 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
4141 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
4201 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
4261 actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct
4321 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
4381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
4621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
4681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
4741 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
4801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
4861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
4921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
4981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5041 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
5101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5221 tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag
5281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
6241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
6301 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
6361 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
6421 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
6481 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
6541 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
6601 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
6661 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
6721 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
6781 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
6841 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
6901 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
6961 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
7021 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
7081 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
7141 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
7201 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
7261 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
7321 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
7381 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
7441 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
7501 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
7561 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
7621 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
7681 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
7741 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
7801 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
7861 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
7921 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
7981 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
8041 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
8101 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
8161 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
8221 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
8281 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
8341 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
8401 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
8461 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
8521 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
8581 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
8641 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
8701 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
8761 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
8821 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
8881 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
8941 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
9001 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
9061 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
9121 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
9181 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
9241 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
9301 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
9361 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
9421 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
9481 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa
9541 attaatacga ctcactatag g
//
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